Purified recombinant E.coli DsbE protein Expression System: E.coli Molecular weight on SDS-PAGE will appear higher.
Reinheit
> 95 % pure
Applikationshinweise
DsbE protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 0 mM EDTA, and 10 % glycerol.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
RT/-20 °C
Informationen zur Lagerung
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Target
DsbE
Hintergrund
DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E. coli and purified by using conventional chromatography. Alternative Names: yejQ protein, Thiol:disulfide interchange protein dsbE Cytochrome c biogenesis protein ccmG..ECK2187 protein, Periplasmic thioredoxin of cytochrome c type biogenesis protein, CcmG protein, JW2183.