Dipeptidyl-Peptidase 4 (DPP4) (AA 39-766) peptide (His tag)
DPP4
Reaktivität: Human
Wirt: Hi-5 Cells
SDS
1 image
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- Target Alle DPP4 Produkte
- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Protein Region
- AA 39-766
- Spezies
- Human
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Quelle
- Hi-5 Cells
- Aufreinigungstag / Konjugat
- Dieses DPP4 Peptid ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- ADP-R KTYTLTDYLK NTYRLKLYSL RWISDHEYLY KQENNILVFN AEYGNSSVFL ENSTFDEFGH SINDYSISPD GQFILLEYNY VKQWRHSYTA SYDIYDLNKR QLITEERIPN NTQWVTWSPV GHKLAYVWNN DIYVKIEPNL PSYRITWTGK EDIIYNGITD WVYEEEVFSA YSALWWSPNG TFLAYAQFND TEVPLIEYSF YSDESLQYPK TVRVPYPKAG AVNPTVKFFV VNTDSLSSVT NATSIQITAP ASMLIGDHYL CDVTWATQER ISLQWLRRIQ NYSVMDICDY DESSGRWNCL VARQHIEMST TGWVGRFRPS EPHFTLDGNS FYKIISNEEG YRHICYFQID KKDCTFITKG TWEVIGIEAL TSDYLYYISN EYKGMPGGRN LYKIQLSDYT KVTCLSCELN PERCQYYSVS FSKEAKYYQL RCSGPGLPLY TLHSSVNDKG LRVLEDNSAL DKMLQNVQMP SKKLDFIILN ETKFWYQMIL PPHFDKSKKY PLLLDVYAGP CSQKADTVFR LNWATYLAST ENIIVASFDG RGSGYQGDKI MHAINRRLGT FEVEDQIEAA RQFSKMGFVD NKRIAIWGWS YGGYVTSMVL GSGSGVFKCG IAVAPVSRWE YYDSVYTERY MGLPTPEDNL DHYRNSTVMS RAENFKQVEY LLIHGTADDN VHFQQSAQIS KALVDVGVDF QAMWYTDEDH GIASSTAHQH IYTHMSHFIK QCFSLP-SGRLVPRGSH HHHHH
- Produktmerkmale
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Purified recombinant Human DPP4 protein
Expression System: Hi-5 cells
Bioactivity: Approximately > 50 Unit/mg. One unit will hydrolyze 1 uM of p-nitroaniline per minute at pH 8.0 and 37 °C using 1mM of Gly-Pro p-nitroanilde as a substrate.
Molecular weight on SDS-PAGE will appear higher. - Reinheit
- > 95 % pure
- Endotoxin-Niveau
- < 1.0 EU per µg of protein (determined by LAL method)
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- Applikationshinweise
- DPP4 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user. Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.
- Testdurchführung
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- Prepare a 80 µL reaction mix in a suitable container: The final concentrations are 1 mM EDTA,21 mM Tris pH8.0, 0.1 M NaCl.
2. Add 10 µL of 11 mM substrate (Gly-Pro p-nitroanilide) to each well.
3. Add 10 µL of recombinant DPP-4 diluent to each well.
4. Incubate the 96 well plate at 37 °C for 10 min.
5. Read the optical density at 405 nm.
- Prepare a 80 µL reaction mix in a suitable container: The final concentrations are 1 mM EDTA,21 mM Tris pH8.0, 0.1 M NaCl.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris-HCl, pH 8.0, containing 100 mM NaCl, 0 mM EDTA, and 10 % glycerol.
- Handhabung
- Avoid repeated freeze/thaw cycles.
- Lagerung
- RT/-20 °C
- Informationen zur Lagerung
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
- Hintergrund
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DPP4, dipeptidyl peptidase-4 is a complex enzyme expressed on the surface of most cell types and is a serine exopeptidase that cleaves x-proline dipeptides from the N-terminus of polypeptides. DPP4 protein is associated with intracellular signal transduction, apoptosis and plays an important role in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). Recombinant human DPP4 protein was expressed with c-terminal His-tag in high-5 cells using baculovirus expression system and purified by using conventional chromatography techniques.
Alternative Names: DPP 4 protein, DPPIV protein, DPP4 protein, DPP4, DPP 4, CD26 protein, ADCP2 protein, dipeptidylpeptidase IV (CD26 adenosine deaminase complexing protein 2) protein, adenosine deaminase complexing protein 2 protein, , TP103 protein, DPP -4 protein, ADABP protein, Dipeptidyl peptidase-4 protein, DPP -4, dipeptidyl-peptidase 4 protein - Molekulargewicht
- 86.4 kDa (746 AA)
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