DnaK (AA 508-638) Peptid
Reaktivität: Alle Spezies
Wirt: Escherichia coli (E. coli)
SDS
Dithiothreitol (DTT)
1 image
Produktnummer ABIN934892
602,66 €
Zzgl. Versandkosten 20,00 € und MwSt
100 μg
Lieferung nach:
Deutschland
Lieferung in 20 bis 27 Werktagen
Kurzübersicht für DnaK (AA 508-638) Peptid (ABIN934892)
Target
DnaK
Spezies
Alle Spezies
Quelle
Escherichia coli (E. coli)
Applikation
SDS-PAGE (SDS)
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Protein-Typ
- Recombinant
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Protein Region
- AA 508-638
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Sequenz
- MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK
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Produktmerkmale
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Purified recombinant E.coli Dnak protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. -
Reinheit
- > 95 % pure
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DnaK (AA 385-546) peptide
Reaktivität: Alle Spezies Wirt: Escherichia coli (E. coli) SDS
DnaK (AA 385-638), (C-Term) peptideReaktivität: Alle Spezies Wirt: Escherichia coli (E. coli) SDS Dithiothreitol (DTT)
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Applikationshinweise
- Dnak protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 25 mM Tris-HCl, pH 7.5, containing 100 mM NaCl, 5 mM DTT, and 10 % glycerol.
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Konservierungsmittel
- Dithiothreitol (DTT)
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Vorsichtsmaßnahmen
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Handhabung
- Avoid repeated freeze/thaw cycles.
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Lagerung
- RT/-20 °C
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Informationen zur Lagerung
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- DnaK
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Hintergrund
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DNAK, originally identified for its DNA replication by bacteriophage in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.
Alternative Names: Dnak (508-638) protein, Heat shock protein 70 protein, HSP70 protein, A lid covering the substrate protein, Chaperone Hsp 70 protein, , Heat shock 70 kDa protein protein, Heat shock 70 kDa protein protein, dnaK protein, Co chaperone with DnaJ protein, Chaperone protein dnaK protein -
Molekulargewicht
- 14.6 kDa (132 AA)
Target
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