DkTx peptide (Atto 647N)
Reaktivität: Cyriopagopus schmidti
Wirt: Synthetic
FACS, FM, IF, LCI
Produktnummer ABIN7885645
1.142,77 €
Zzgl. Versandkosten 20,00 € und MwSt
Lieferung nach:
Deutschland
Lieferung in 8 bis 9 Werktagen
Kurzübersicht für DkTx peptide (Atto 647N) (ABIN7885645)
Target
DkTx
Spezies
Cyriopagopus schmidti
Quelle
Synthetic
Applikation
Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Protein-Typ
- Synthetic
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Biologische Aktivität
- Active
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Aufreinigungstag / Konjugat
- Atto 647N
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Verwendungszweck
- DkTx-ATTO Fluor-647N
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Sequenz
- DCAKEGEVCS WGKKCCDLDN FYCPMEFIPH CKKYKPYVPV TTNCAKEGEV CGWGSKCCHG LDCPLAFIPY CEKYR-OH
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Spezifität
- Toxin target: TRPV1 channel
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Produktmerkmale
- ATTO 647N (Ex/Em: 646/664 nm). The extent of labeling is one molecule of dye per one DkTx molecule, linked via primary amines.
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Biological Activity Comment
- Selectively activates the heat-activated TRPV1 channel1,2.
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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Handhabung
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light
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Lagerung
- -20 °C
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Informationen zur Lagerung
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.
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- DkTx
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Hintergrund
- A fluorescent probe for imaging and sensitive detection of TRPV1 Channel
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Molekulargewicht
- 9155 Da
Target
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