Endothelin 1 (EDN1) Peptid
EDN1
Wirt: Synthetic
BP, Func
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- Target Alle Endothelin 1 (EDN1) Produkte
- Endothelin 1 (EDN1)
- Protein-Typ
- Synthetic
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Quelle
- Synthetic
- Applikation
- Blocking Peptide (BP), Functional Studies (Func)
- Verwendungszweck
- A Potent Agonist of Endothelin B Receptors
- Sequenz
- ASASSLMDKE AVYFAHLDII W-OH
- Spezifität
- Toxin target: ET-B receptor
- Produktmerkmale
- A Potent Agonist of Endothelin B Receptors
- Reinheit
- ≥98 % (HPLC)
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- Applikationshinweise
- Antigen Preadsorption Control: 1 pM - 100 nM
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. Avoid multiple freeze-thaw cycles to maintain biological activity. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1 % -0.5 % DMSO (v/v) is considered safe. For other experiments, a 5 % DMSO (v/v) concentration is recommended.
- Buffer
- Lyophilized Powder.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day.,Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.
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- Target
- Endothelin 1 (EDN1)
- Hintergrund
- [Ala1,3,11,15 ] Endothelin-1 is a potent ET-B (endothelin B) receptor agonist, a receptor that belongs to the G-protein coupled receptor (GPCR) superfamily. [Ala1,3,11,15] Endothelin-1 is a linear synthetic peptide analog of endothelin 1 (ET-1) that shows very high affinity for the receptor. The analog includes a substitution of Ala residue for Cys residues that removes the disulfide bridges that exist in endothelin-11-3.Endothelin-1 (ET-1) and its receptor ET-B play an important role in regulating renal function and blood pressure. ET-B Receptor and ET-1 are both expressed in sensory nerves. They are mainly present in medium- and large-sized cell bodies of human trigeminal ganglia1.Studies have shown that acute administration of an ET-B receptor agonist into the rat renal medulla leads to natriuresis and diuresis via a mechanism that is NOS1-dependent3.
- Molekulargewicht
- 2367.7 Da
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