Conotoxin MVIIC omega Peptid
Reaktivität: Conus magus (Magical cone)
Wirt: Synthetic
BP, FACS, FM, IF, LCI
Produktnummer ABIN7582092
Kurzübersicht für Conotoxin MVIIC omega Peptid (ABIN7582092)
Target
Conotoxin MVIIC omega
Spezies
Conus magus (Magical cone)
Quelle
Synthetic
Applikation
Blocking Peptide (BP), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Protein-Typ
- Synthetic
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Sequenz
- CKGKGAPCRK TMYDCCSGSC GRRGKC-NH2
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Spezifität
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Toxin target: N-type and P/Q-type Ca2+ channels,
Disulfide bonds between: Cys1-Cys16, Cys8-Cys20 and Cys15-Cys26Cys26 - C-terminal amidation ATTO-Fluor 647N -
Produktmerkmale
- A Blocker of Presynaptic CaV (N- and P/Q-Type) Channels
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Conotoxin MVIIC omega peptide (Biotin)
Reaktivität: Conus magus (Magical cone) Wirt: Synthetic BP, FACS, FM, IF, LCI
Conotoxin MVIIC omega peptideReaktivität: Conus magus (Magical cone) Wirt: Synthetic BP
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Applikationshinweise
- Antigen Preadsorption Control: 0.1 - 1 μM
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 mL conical vial. The product is soluble in pure water to high-micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.,Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
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- Conotoxin MVIIC omega
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Hintergrund
- SNX-230, Omega-CgTxMVIIC, Omega-conotoxin MVIIC,ω-Conotoxin MVIIC blocks CaV2.1 (α1A, P/Q-type) and CaV2.2 (α1B, N-type) channels.1 The toxin binds with high affinity to CaV2.1 and with lower affinity to CaV2.2 in rabbit brain.2 However, the block by ω-Conotoxin-MVIIC of N-type channels in DRG neurons developed much faster than the block of P-type currents in Purkinje cells.1 The effect of the toxin is modulated by voltage (i.e. it is more potent for inactivated channels).3 In addition, this toxin was reported to block nicotinic receptors (transiently expressed in Xenopus oocytes) with IC50 of 1.3 μM.4 It was also shown to inhibit K+-induced 3H-GABA release in hippocampus in vivo.5 This effect was with high affinity (50 % block, 200 nM). The toxin was used to inhibit synaptic transmission in several peripheral preparations.6,7
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Molekulargewicht
- 3376 Da
Target
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