Stratifin (SFN) Peptid
SFN
Reaktivität: Human
Wirt: Synthetic
BP, BI
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- Target Alle 14-3-3 sigma/SFN (SFN) Produkte
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
- Spezies
- Human
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Quelle
- Synthetic
- Applikation
- Blocking Peptide (BP), BioImaging (BI)
- Produktmerkmale
- 14-3-3 sigma peptide corresponds to 14 amino acids of 14-3-3 sigma.
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- Applikationshinweise
- 14-3-3 sigma Peptide is used for blocking the activity of 14-3-3 sigma antibody . The 14-3-3 sigma antibody ships at 0.5 mg/mL and the 14-3-3 sigma Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- 14-3-3 sigma peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C. When reconstituted minimize freezing and thawing.
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