When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 19 kDa band observed in Mouse fetal Brain lysates (calculated MW of 19.0 kDa according to NP_032767.1). Recommended concentration: 0.1-0.3 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
NSG2 (HMP19)
(Neuron-Specific Protein Family Member 2 (HMP19))
Synonyme
NSG2 Peptide, 8.5 Peptide, AA989750 Peptide, R75287 Peptide, neuronal vesicle trafficking associated 2 Peptide, neuron specific gene family member 2 Peptide, NSG2 Peptide, Nsg2 Peptide