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Protein Kinase D1 (PRKD1) (AA 383-395), (Internal Region) Peptid

PRKD1 Reaktivität: Human Wirt: Synthetic BP, Imm
Produktnummer ABIN5539402

Kurzübersicht für Protein Kinase D1 (PRKD1) (AA 383-395), (Internal Region) Peptid (ABIN5539402)

Target

PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Spezies

Human

Quelle

  • 7
Synthetic

Applikation

Blocking Peptide (BP), Immunogen (Imm)
  • Protein Region

    AA 383-395, Internal Region

    Sequenz

    QDPDPDHEDA NRT

    Homologie

    Human
  • Applikationshinweise

    When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100 kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or PKD3. Recommen

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.

    Buffer

    100µg of Lyophilised peptide, formulation De-ionized (DI) water.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Shipped at ambient temperature, store at -20°C.
  • Target

    PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

    Gen-ID

    5587

    NCBI Accession

    NP_002733
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