When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 40-45 kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 46.6 kDa according to NP_001193.2). Recommended concentration: 0.3-1 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
BMP4
(Bone Morphogenetic Protein 4 (BMP4))
Synonyme
BMP2B Peptide, BMP2B1 Peptide, MCOPS6 Peptide, OFC11 Peptide, ZYME Peptide, Bmp-4 Peptide, Bmp2b Peptide, Bmp2b-1 Peptide, Bmp2b1 Peptide, BOMPR4A Peptide, bmp-4 Peptide, zbmp-4 Peptide, zgc:100779 Peptide, BMP-4 Peptide, XBMP-4 Peptide, bmp2b Peptide, bmp2b1 Peptide, bmp4 Peptide, ofc11 Peptide, xbmp4 Peptide, zyme Peptide, BMP4 Peptide, bone morphogenetic protein 4 Peptide, bone morphogenetic protein 4 L homeolog Peptide, bone morphogenetic protein 4 S homeolog Peptide, BMP4 Peptide, Bmp4 Peptide, bmp4 Peptide, bmp4.L Peptide, bmp4.S Peptide