When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
FBXL3
(F-Box and Leucine-Rich Repeat Protein 3 (FBXL3))
Synonyme
FBL3 Peptide, FBL3A Peptide, FBXL3A Peptide, AU041772 Peptide, AW212966 Peptide, FBK Peptide, Fbl3a Peptide, Fbxl3a Peptide, Ovtm Peptide, Play68 Peptide, fbxl3 Peptide, FBXL3 Peptide, F7A7.240 Peptide, F7A7_240 Peptide, im:7153024 Peptide, zgc:103721 Peptide, F-box and leucine rich repeat protein 3 Peptide, F-box and leucine-rich repeat protein 3 Peptide, F-box and leucine-rich repeat protein 3 L homeolog Peptide, RNI-like superfamily protein Peptide, F-box and leucine-rich repeat protein 3a Peptide, FBXL3 Peptide, Fbxl3 Peptide, fbxl3.L Peptide, fbxl3 Peptide, AT5G01720 Peptide, fbxl3a Peptide