When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
FHL1
(Four and A Half LIM Domains 1 (FHL1))
Synonyme
fhl1 Peptide, MGC89165 Peptide, FHL1 Peptide, DKFZp468O1927 Peptide, fhl Peptide, fhla Peptide, zgc:92025 Peptide, FHL-1 Peptide, FHL1A Peptide, FHL1B Peptide, FLH1A Peptide, KYOT Peptide, SLIM Peptide, SLIM-1 Peptide, SLIM1 Peptide, SLIMMER Peptide, XMPMA Peptide, KyoT Peptide, Fhl1 Peptide, four and a half LIM domains 1 Peptide, four and a half LIM domains 1a Peptide, Four and a half LIM domains protein 1 Peptide, four and a half LIM domains 1 S homeolog Peptide, fhl1 Peptide, FHL1 Peptide, fhl1a Peptide, fhl1.S Peptide, Fhl1 Peptide