When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
GABPB2
(GA-Binding Protein Subunit beta-2 (GABPB2))
Synonyme
GABP2 Peptide, GABPB1 Peptide, 1810015F01Rik Peptide, 5830427M07 Peptide, 9430006E19Rik Peptide, A430024B14Rik Peptide, AV050852 Peptide, Gabpb2-1 Peptide, RGD1565234 Peptide, gabpb2 Peptide, wu:fi15e02 Peptide, wu:fi17h09 Peptide, zgc:55805 Peptide, ankhd1 Peptide, GABPB2 Peptide, GABPAP Peptide, im:7142141 Peptide, si:dkey-77n11.3 Peptide, zgc:154003 Peptide, GA binding protein transcription factor beta subunit 2 Peptide, GA repeat binding protein, beta 2 Peptide, GA binding protein transcription factor, beta subunit 2 Peptide, GA binding protein transcription factor, beta subunit 2a Peptide, GA binding protein transcription factor beta subunit 2 S homeolog Peptide, GA binding protein transcription factor beta subunit 1 Peptide, GA binding protein transcription factor, beta subunit 2b Peptide, GABPB2 Peptide, Gabpb2 Peptide, gabpb2a Peptide, gabpb2.S Peptide, GABPB1 Peptide, gabpb2b Peptide