When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
RBP4
(Retinol Binding Protein 4, Plasma (RBP4))
Synonyme
RDCCAS Peptide, RBP Peptide, RNBP Peptide, rbp4 Peptide, MGC54038 Peptide, srbp Peptide, PRBP Peptide, RBPA Peptide, Rbp-4 Peptide, fb23c12 Peptide, rbp Peptide, wu:fb23c12 Peptide, wu:fb58d04 Peptide, wu:fb72b04 Peptide, zgc:86911 Peptide, retinol binding protein 4 Peptide, renin binding protein Peptide, retinol binding protein 4 S homeolog Peptide, retinol binding protein 4 A, plasma Peptide, retinol binding protein 4, plasma Peptide, retinol binding protein 4 L homeolog Peptide, RBP4 Peptide, RENBP Peptide, rbp4.S Peptide, rbp4 Peptide, Rbp4 Peptide, RBP4A Peptide, rbp4.L Peptide