When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
VPS11
(Vacuolar Protein Sorting 11 Homolog (VPS11))
Synonyme
zgc:112329 Peptide, ATVPS11 Peptide, F5G3.7 Peptide, F5G3_7 Peptide, vacuolar protein sorting 11 Peptide, DDBDRAFT_0204454 Peptide, DDBDRAFT_0234135 Peptide, DDB_0204454 Peptide, DDB_0234135 Peptide, end1 Peptide, pep5 Peptide, hvps11 Peptide, rnf108 Peptide, 1200011A11Rik Peptide, END1 Peptide, PEP5 Peptide, RNF108 Peptide, hVPS11 Peptide, VPS11, CORVET/HOPS core subunit Peptide, vacuolar protein sorting 11 Peptide, vacuolar protein sorting protein 11 Peptide, VPS11, CORVET/HOPS core subunit S homeolog Peptide, Vacuolar peripheral membrane protein similar to S. cerevisiae PEP5 (YMR231W); C terminus is almost identical to nonallelic CaP19.1278 Peptide, 7-fold repeat in clathrin and VPS proteins repeat-containing protein Peptide, vacuolar protein sorting-associated protein 11 homolog Peptide, VPS11 CORVET/HOPS core subunit Peptide, VPS11 Peptide, vps11 Peptide, MCYG_02909 Peptide, vps11.S Peptide, LOC100632536 Peptide, Vps11 Peptide