When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
UCP1
(Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))
Synonyme
ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1 Peptide, ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1 Peptide, ATPUMP1 Peptide, ATUCP1 Peptide, UCP Peptide, UCP1 Peptide, UNCOUPLING PROTEIN 1 Peptide, plant uncoupling mitochondrial protein 1 Peptide, ucp Peptide, slc25a7 Peptide, SLC25A7 Peptide, AI385626 Peptide, Slc25a7 Peptide, Ucp Peptide, Ucpa Peptide, Uncp Peptide, plant uncoupling mitochondrial protein 1 Peptide, uncoupling protein 1 Peptide, uncoupling protein 1 L homeolog Peptide, uncoupling protein 1 (mitochondrial, proton carrier) Peptide, PUMP1 Peptide, UCP1 Peptide, ucp1.L Peptide, ucp1 Peptide, Ucp1 Peptide