When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
CAMK1D
(Calcium/calmodulin-Dependent Protein Kinase ID (CAMK1D))
Synonyme
CKLiK Peptide, CaM-K1 Peptide, CaMKID Peptide, A630059D12Rik Peptide, CaMKIdelta Peptide, E030025C11Rik Peptide, CAMK1D Peptide, camk1d Peptide, zgc:158713 Peptide, RGD1560691 Peptide, zgc:172284 Peptide, cam-ki Peptide, camk1d.L Peptide, calcium/calmodulin dependent protein kinase ID Peptide, calcium/calmodulin-dependent protein kinase ID Peptide, calcium/calmodulin-dependent protein kinase 1Db Peptide, calcium/calmodulin-dependent protein kinase 1Da Peptide, calcium/calmodulin dependent protein kinase ID S homeolog Peptide, CAMK1D Peptide, Camk1d Peptide, camk1db Peptide, LOAG_08089 Peptide, camk1da Peptide, camk1d.S Peptide