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DYKDDDDK Tag Peptid

Wirt: Synthetic IP, SDS, WB Without preservative
Rockland
Produktnummer ABIN1607596
Hersteller Produkt- Nr.: 000-000-h47

Kurzübersicht für DYKDDDDK Tag Peptid (ABIN1607596)

Target

DYKDDDDK Tag

Quelle

  • 1
Synthetic

Applikation

Immunoprecipitation (IP), SDS-PAGE (SDS), Western Blotting (WB)
  • Protein-Typ

    Synthetic

    Hersteller Produkt- Nr.

    000-000-h47

    Hersteller

    Rockland

    Verwendungszweck

    Triple FLAG® Peptide

    Produktmerkmale

    synthetic FLAG® triple peptide, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide, control peptide, blocking peptide

    Aufreinigung

    Greater than 90% specific peptide by HPLC and MS.

    Reinheit

    Greater than 90% specific peptide by HPLC and MS.
  • Applikationshinweise

    Application Note: The Triple FLAG® Peptide has been tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG® peptide is typically used for competitive elution of FLAG® containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 μg per 1.0 μL of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG® affinity gel PROTOCOL for recommendations for use.

    Western Blot Dilution: YES as blocking peptide in PCA

    Immunoprecipitation Dilution: YES - see PROTOCOL

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4

    Reconstitution Volume: 1.0 mL

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: None

    Stabilizer: None

    Preservative: None

    Konservierungsmittel

    Without preservative

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store vial at -20 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

    Haltbarkeit

    6 months
  • Target

    DYKDDDDK Tag

    Substanzklasse

    Tag

    Hintergrund

    Synonyms: synthetic FLAG® triple peptide, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide, control peptide, blocking peptide

    Background: The Triple FLAG® Peptide is a synthetic peptide of 23 amino acid residues (molecular weight of 2861.9 daltons). The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG® sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys or DYKDDDDK). The triple FLAG peptide is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free triple FLAG® Peptide.

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