PCDHG Antikörper (C-Term, Cytoplasmic Domain, pan)
Der Maus Monoklonal Anti-PCDHG-Antikörper wurde für ICC, IF und WB validiert. Er ist geeignet, PCDHG in Proben von Maus und Ratte zu detektieren.
Produktnummer ABIN5539938
Kurzübersicht für PCDHG Antikörper (C-Term, Cytoplasmic Domain, pan) (ABIN5539938)
Target
PCDHG
(Protocadherin gamma Cluster (PCDHG))
Reaktivität
Maus, Ratte
Wirt
Maus
Klonalität
Monoklonal
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Klon
S159-5
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Bindungsspezifität
- AA 808-931, C-Term, Cytoplasmic Domain, pan
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Spezifität
- This antibody detects a ~100 kDa protein. Cross-reacts with all Gamma- protocadherin-A, -B and -C proteins. Species: Mouse and Rat.
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Aufreinigung
- Protein G Chromatography
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Immunogen
- Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A-subfamily: amino acids ~807-930, B-subfamily: amino acids ~789-912 and C subfamily).
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Isotyp
- IgG1
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Applikationshinweise
- Western blot: 1/1000, 1 μg/mL was sufficient for detection of PCDHG (pan) in 20 μg of Rat brain lysate by colorimetric immunoblot analysis using HRP conjugated secondary antibody. Immunocytochemistry .
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- PBS pH 7.4, 50 % Glycerol, 0.09 % Sodium Azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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- PCDHG (Protocadherin gamma Cluster (PCDHG))
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Andere Bezeichnung
- pcdhg
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Hintergrund
- The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
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Gen-ID
- 93709
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UniProt
- Q91XZ0
Target
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