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Maus CXCL4/PF4 EZ Set™ ELISA Kit (DIY Antibody Pairs)

ELISA Reaktivität: Maus ELISA unconjugated AA 30-105 Wirt: Kaninchen
Produktnummer ABIN5510776
  • Target Alle PF4 Produkte
    PF4 (Platelet Factor 4 (PF4))
    Bindungsspezifität
    AA 30-105
    Reaktivität
    Maus
    Nachweismethode
    Colorimetric
    Wirt
    • 1
    • 1
    Kaninchen
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CXCL4/PF4 in Mouse samples.
    Marke
    EZ-Set™
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: V30-S105
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Bestandteile
    Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
    Benötigtes Material
    1. Microplate reader in standard size.
    2. Automated plate washer.
    3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
    4. Clean tubes and Eppendorf tubes.
    5. Washing buffer (neutral PBS or TBS).
  • Kommentare

    The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
    The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
    The EZ Set™ Accessory Kit provides:
    Antibody diluent buffer
    ABC diluent buffer
    Sample diluent buffer
    TMB
    TMB stop solution
    5x 96 well plates, plate sealers

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
    Haltbarkeit
    12 months
  • Target
    PF4 (Platelet Factor 4 (PF4))
    Andere Bezeichnung
    Pf4 (PF4 Produkte)
    Synonyme
    CXCL4 Matched Antibody Pair, PF-4 Matched Antibody Pair, SCYB4 Matched Antibody Pair, Cxcl4 Matched Antibody Pair, Scyb4 Matched Antibody Pair, Pf4a Matched Antibody Pair, RATPF4A Matched Antibody Pair, PF4A Matched Antibody Pair, CXCL4L1 Matched Antibody Pair, CXCL4V1 Matched Antibody Pair, PF4-ALT Matched Antibody Pair, SCYB4V1 Matched Antibody Pair, PF4 Matched Antibody Pair, platelet factor 4 Matched Antibody Pair, platelet factor 4 variant 1 Matched Antibody Pair, PF4 Matched Antibody Pair, Pf4 Matched Antibody Pair, LOC703451 Matched Antibody Pair, PF4V1 Matched Antibody Pair, LOC100524373 Matched Antibody Pair, LOC102181854 Matched Antibody Pair, LOC100630489 Matched Antibody Pair
    Hintergrund

    Protein Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity). .

    Background: Platelet factor 4(PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine(C-X-C motif) ligand 4(CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

    Synonyms: Platelet factor 4,PF-4,C-X-C motif chemokine 4,Pf4,Cxcl4, Scyb4,

    Full Gene Name: Platelet factor 4

    Cellular Localisation: Secreted.
    UniProt
    Q9Z126
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