Human CD40/TNFRSF5 EZ Set™ ELISA Kit (DIY Antibody Pairs)
Kurzübersicht für Human CD40/TNFRSF5 EZ Set™ ELISA Kit (DIY Antibody Pairs) (ABIN5510774)
Target
Bindungsspezifität
Reaktivität
Wirt
Applikation
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Nachweismethode
- Colorimetric
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Methodentyp
- Sandwich ELISA
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Verwendungszweck
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CD40 in Human samples.
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Spezifität
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Expression system for standard: NSO
Immunogen sequence: E21-R193 -
Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Produktmerkmale
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Sequence similarities: Contains 4 TNFR-Cys repeats.
Tissue Specificity: B-cells and in primary carcinomas.
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Bestandteile
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
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Benötigtes Material
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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CD40 (Human) Matched Antibody Pair
ELISA Reaktivität: Human ELISA unconjugated Wirt: Maus
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Kommentare
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. -
Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Haltbarkeit
- 12 months
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- CD40
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Andere Bezeichnung
- CD40
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Hintergrund
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Protein Function: Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
Synonyms: Tumor necrosis factor receptor superfamily member 5,B-cell surface antigen CD40,Bp50,CD40L receptor,CDw40,CD40,CD40,TNFRSF5,
Full Gene Name: Tumor necrosis factor receptor superfamily member 5
Cellular Localisation: Isoform I: Cell membrane, Single-pass type I membrane protein. -
UniProt
- P25942
Target
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