Human OSM/Oncostatin M EZ Set™ ELISA Kit (DIY Antibody Pairs)
Kurzübersicht für Human OSM/Oncostatin M EZ Set™ ELISA Kit (DIY Antibody Pairs) (ABIN5510771)
Target
Reaktivität
Wirt
Applikation
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Nachweismethode
- Colorimetric
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Methodentyp
- Sandwich ELISA
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Verwendungszweck
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects OSM/Oncostatin M in Human samples.
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Marke
- EZ-Set™
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Spezifität
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Expression system for standard: E.coli
Immunogen sequence: A26R-220 -
Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Bestandteile
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
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Benötigtes Material
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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OSM (Human) IP-WB Antibody Pair
IP Reaktivität: Human IP unconjugated Wirt: Kaninchen, Maus
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Kommentare
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. -
Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Haltbarkeit
- 12 months
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- Oncostatin M (OSM)
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Andere Bezeichnung
- OSM
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Hintergrund
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Protein Function: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity). .
Background: OSM(Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor(LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.
Synonyms: Oncostatin-M,OSM,OSM,
Full Gene Name: Oncostatin-M
Cellular Localisation: Secreted. -
UniProt
- P13725
Target
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