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Maus IL-10 ELISPOT Pair

ELISpot Reaktivität: Maus ELISpot unconjugated
Produktnummer ABIN2691017
  • Target Alle IL-10 (IL10) Produkte
    IL-10 (IL10) (Interleukin 10 (IL10))
    Reaktivität
    Maus
    Applikation
    ELISpot
    Marke
    BD™ ELISPOT
    Sterilität
    0.2 μm filtered
    Endotoxin-Niveau
    Endotoxin level is ≤0.01 ng/μg of protein.
    Bestandteile
    This product contains sufficient reagent for five 96-well plates, including unlabelled capture antibody (no azide/low endotoxin format), biotinylated detection antibody, and a Certificate of Analysis that provides lot-specific optimal reagent concentrations.
  • Applikationshinweise
    The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis.
    Kommentare

    BD™ ELISPOT Mouse IL-10 ELISPOT Pair

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm filtered.
    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
  • Fujihashi, McGhee, Beagley, McPherson, McPherson, Huang, Kiyono: "Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 181-9, (1993) (PubMed).

    Czerkinsky, Andersson, Ekre, Nilsson, Klareskog, Ouchterlony: "Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells." in: Journal of immunological methods, Vol. 110, Issue 1, pp. 29-36, (1988) (PubMed).

    Sedgwick, Holt: "A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 57, Issue 1-3, pp. 301-9, (1983) (PubMed).

  • Target
    IL-10 (IL10) (Interleukin 10 (IL10))
    Andere Bezeichnung
    IL-10 (IL10 Produkte)
    Synonyme
    CSIF Matched Antibody Pair, GVHDS Matched Antibody Pair, IL-10 Matched Antibody Pair, IL10A Matched Antibody Pair, TGIF Matched Antibody Pair, Il-10 Matched Antibody Pair, IL10X Matched Antibody Pair, interleukin 10 Matched Antibody Pair, IL10 Matched Antibody Pair, Il10 Matched Antibody Pair
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