Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) Antikörper
Der Ziege Polyklonal Anti--Antikörper wurde für ELISA, FACS, IF und WB validiert. Er ist geeignet, in Proben von Human zu detektieren.
Produktnummer ABIN185294
Kurzübersicht für Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) Antikörper (ABIN185294)
Target
Alle Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) Produkte
Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
Reaktivität
Human
Wirt
Ziege
Klonalität
Polyklonal
Applikation
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
Güteklasse
Verified
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Bindungsspezifität
- AA 429-442, AA 443-456
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Verwendungszweck
- LXR alpha / LXR beta
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Sequenz
- CRLQDKKLPP LLSEI
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Spezifität
- This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
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Kreuzreaktivität
- Rind (Kuh), Hund, Human, Maus, Ratte
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Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.
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Isotyp
- IgG
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Applikationshinweise
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Western Blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing pe
Peptide ELISA: antibody detection limit dilution 1:32000.
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Kommentare
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Minimize freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
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Andere Bezeichnung
- NR1H3, NR1H2
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Hintergrund
- NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N
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Gen-ID
- 10062, 7376
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NCBI Accession
- NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524
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Pathways
- Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
Target
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