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Microrchidia 2A Protein (MORC2A) (AA 1-1030) (Strep Tag)

Dieses Recombinant Microrchidia 2A-Protein wird in Cell-free protein synthesis (CFPS) produziert.
Produktnummer ABIN3123434
ab
15.754,29 €
Zzgl. Versandkosten 20,00 €, Trockeneispauschale 20,00 € sowie MwSt
250 μg
Lieferung nach: Deutschland
Lieferung in 51 bis 61 Werktagen

Kurzübersicht für Microrchidia 2A Protein (MORC2A) (AA 1-1030) (Strep Tag) (ABIN3123434)

Target

Microrchidia 2A (MORC2A)

Protein-Typ

Recombinant

Spezies

Maus

Quelle

Cell-free protein synthesis (CFPS)

Applikation

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Reinheit

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse

custom-made
  • Proteineigenschaft

    AA 1-1030

    Aufreinigungstag / Konjugat

    Dieses Microrchidia 2A Protein ist gelabelt mit Strep Tag.

    Sequenz

    MAFTNYSSLN RAQLTFEYLH TNSTTHEFLF GALAELVDNA RDADATRIDI YAERREDLRG GFMLCFLDDG AGMDPSDAAS VIQFGKSAKR TPESTQIGQY GNGLKSGSMR IGKDFILFTK KEDTMTCLFL SRTFHEEEGI DEVIVPLPTW NARTREPITD NVEKFAIETE LVYKYSPFHT EEQVMNQFMK IPGNSGTLVI IFNLKLMDNG EPELDIISNP KDIQMAETSP EGTKPERRSF RAYAAVLYID PRMRIFIHGH KVQTKRLSCC LYKPRMYKYT SSRFKTRAEQ EVKKAEHVAR IAEEKAREAE SKARTLEVRM GGDLTRDSRV MLRQVQNTAI TLRREADVKK RIKDAKQRAL KEPKELNFVF GVNIEHRDLD GMFIYNCSRL IKMYEKVGPQ LEGGMACGGV VGVVDVPYLV LEPTHNKQDF ADAKEYRHLL RAMGEHLAQY WKDIAIAQRG IIKFWDEFGY LSANWNQPPS SELRFKRRRA MEIPTTIQCD LCLKWRTLPF QLSSVETDYP DTWVCSMNPD PEQDRCEASE QKQKVPLGTL KKDPKTQEEK QKQLTEKIRQ QQEKLEALQK TTPIRSQADL KKLPLEVTTR PIEEPVRRPQ RPRSPPLPAV IKNAPSRPPS IQTPRPSTQL RKTSVISLPK PPTTAARGET STSRLLQPTE APRKPANPPI KTVPRPTPPV HTPPLSLIPS SKSLREVPAQ KAIKTPVVKK PEPPVKQSVA TSGRKRSLAV SDEEEAEEEA EKRRERCKRG KLAVKEEKKE ANELSDSAGE DHPAELRKAQ KDKGLHVEVR VNREWYTGRV TAVEVGKNAV RWKVKFDYVP TDTTPRDRWV EKGSEDVRLM KPPSPEHQSP DTQQEGGEEE EAMVARQAVA LPEPSTSDGL PIEPDTTATS PSHETIDLLV QILRNCLRYF LPPSFPISKK ELSVMNSEEL ISFPLKEYFK QYEVGLQNLC HSYQSRADSR AKASEESLRT SEKKLRETEE KLQKLRTNIV ALLQKVQEDI DINTDDELDA YIEDLITKGD
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    Produktmerkmale

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Aufreinigung

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • Applikationshinweise

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    Handhabung

    Avoid repeated freeze-thaw cycles.

    Lagerung

    -80 °C

    Informationen zur Lagerung

    Store at -80°C.

    Haltbarkeit

    12 months
  • Target

    Microrchidia 2A (MORC2A)

    Andere Bezeichnung

    Morc2a

    Hintergrund

    ATPase MORC2A (EC 3.6.1.-) (MORC family CW-type zinc finger protein 2A) (Zinc finger CW-type coiled-coil domain protein 1),FUNCTION: Essential for epigenetic silencing by the HUSH complex. Recruited by HUSH to target site in heterochromatin, the ATPase activity and homodimerization are critical for HUSH-mediated silencing (By similarity). Represses germ cell-related genes and L1 retrotransposons in collaboration with SETDB1 and the HUSH complex, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark (PubMed:29728365). Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression. During DNA damage response, regulates chromatin remodeling through ATP hydrolysis (By similarity). During DNA damage response, may regulate chromatin remodeling through ATP hydrolysis (By similarity). {ECO:0000250|UniProtKB:Q9Y6X9, ECO:0000269|PubMed:29728365}.

    Molekulargewicht

    117.3 kDa

    UniProt

    Q69ZX6
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