anti-CDT1 (CDT1) Antikörper

Zebrafish Chromatin Licensing and DNA Replication Factor 1 (CDT1) Interaktionspartner

1. DTL (zeige DTL Antikörper) promotes genomic stability through two distinct mechanisms. First, it is an essential component of the CUL4-DDB1 complex that controls CDT1 levels, thereby preventing rereplication. Second, it is required for the early G2/M checkpoint.

Human Chromatin Licensing and DNA Replication Factor 1 (CDT1) Interaktionspartner

1. PPP2R3B (zeige PPP2R3B Antikörper) codes for the PR70 protein, a regulatory substrate-recognizing subunit of protein phosphatase 2A. PR70 decreased melanoma growth by negatively interfering with DNA replication and cell cycle progression through its role in stabilizing CDC6 (zeige CDC6 Antikörper)-chromatin licensing and CDT1 interaction

2. USP37 (zeige USP37 Antikörper) interacts with Cdt1 and is able to de-ubiquitinate Cdt1 in vivo and, USP37 (zeige USP37 Antikörper) is able to regulate the loading of MCM complexes onto the chromatin.

3. mismatch repair (MMR (zeige MRC1 Antikörper)) proteins are also involved in the degradation of Cdt1 after ultraviolet irradiation in the G1 phase

4. Cdt1-binding protein GRWD1 promotes chromatin fluidity by influencing nucleosome structures, e.g., by transient eviction of H2A-H2B, and thereby promotes efficient MCM loading at replication origins.

5. ATM (zeige ATM Antikörper) silencing induced partial reduction in levels of Skp2, a component of SCF (zeige KITLG Antikörper)(Skp2) ubiquitin ligase that controls Cdt1 degradation.

6. FBXO31 (zeige FBXO5 Antikörper) interacts with Cdt1 and regulates the degradation of Cdt1 in G2 phase.

7. Protein levels of Geminin (zeige GMNN Antikörper) and Cdt1 are tightly regulated through the cell cycle, and the Cdt1-Geminin (zeige GMNN Antikörper) complex likely acts as a molecular switch that can enable or disable the firing of each origin of replication.

8. These results demonstrate an important role for Cdt1 in human papillomavirus E7-induced rereplication and shed light on mechanisms by which human papillomavirus induces genomic instability.

9. ATR (zeige ANTXR1 Antikörper), activated after DNA damage, phosphorylates Cdt2 (zeige DTL Antikörper) and promotes the rapid degradation of Cdt1 after UV irradiation in the G1 phase of the cell cycle.

10. A lethal phenotype was seen in four individuals with compound heterozygous CDT1 mutations

Mouse (Murine) Chromatin Licensing and DNA Replication Factor 1 (CDT1) Interaktionspartner

1. conserved arginine residues play critical roles in interaction with Geminin (zeige GMNN Antikörper) and Mcm that are crucial for proper conformation of the complexes and its licensing activity.

2. Study shows that ablation of Geminin (zeige GMNN Antikörper) induces massive rereplication as a result of unrestrained Cdt1 activity in embryonic stem cells, whereas it has no such effect in embryonic fibroblasts in which alternative regulation of Cdt1 activity is intact.

3. This study reveals a conserved new regulatory Cdt1 domain crucial for proper DNA licensing activity.

4. Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase (zeige DNA2 Antikörper) Mcm proteins.

5. Determined the structures of mCdt1CS (mCdt1C_small; residues 452 to 557) and mCdt1CL (mCdt1C_large; residues 420 to 557) using X-ray crystallography and NMR. This study reveals that Cdt1 is formed with a tandem repeat of the winged helix domain.

6. Cdt1 function is negatively regulated by the Cdk (zeige CDK4 Antikörper) phosphorylation independent of geminin (zeige GMNN Antikörper) binding

7. crystal structure of the mouse geminin (zeige GMNN Antikörper)-Cdt1 complex using tGeminin (residues 79-157, truncated geminin (zeige GMNN Antikörper)) and tCdt1 (residues 172-368, truncated Cdt1)

8. In situ hybridization and immunohistochemistry localize Cdt1 as well as geminin (zeige GMNN Antikörper) to the proliferative compartment of the developing mouse gut (zeige GUSB Antikörper) epithelium

9. Cdt1 expression characterizes progenitor cells in G1 phase

10. These results suggested that, at least in vitro, oleic acid-containing cell membranes of the lipid bilayer inhibit Cdt1-geminin (zeige GMNN Antikörper) complex formation by binding to Cdt1 and thereby liberating Cdt1 from inhibition by geminin (zeige GMNN Antikörper).

Xenopus laevis Chromatin Licensing and DNA Replication Factor 1 (CDT1) Interaktionspartner

1. The results showed that the Cdt1 region spanning amino acids (a. a.) 255-620 is required for efficient inhibition of DNA replication, and that, within this region, a. a. 255-289 have a critical role in inhibition.

2. conserved arginine residues play critical roles in interaction with Geminin (zeige GMNN Antikörper) and Mcm that are crucial for proper conformation of the complexes and its licensing activity.

3. These findings suggested that excess Cdt1 suppressed the progression of replication forks.

4. Dynamic interactions of high Cdt1 and geminin (zeige GMNN Antikörper) levels regulate S phase in early Xenopus embryos.

5. p97 (zeige vcp Antikörper) is an essential regulator of DNA damage-dependent CDT1 destruction

6. CDC-48/p97 (zeige vcp Antikörper) coordinates CDT-1 degradation with GINS chromatin dissociation to ensure faithful DNA replication

7. Loading of geminin (zeige GMNN Antikörper) onto chromatin requires Cdt1, suggesting that geminin (zeige GMNN Antikörper) is targeted at replication origins.

8. results allow us to build a comprehensive model of how re-replication of DNA is prevented in Xenopus, with Cdt1 regulation being the key feature

9. Removal of Cdt1 from chromatin and its nuclear exclusion in G2 is critical in regulating licensing and limiting DNA replication in S phase to only one round.

10. Cdt1 and DDB1 interact in extracts, and DDB1 chromatin loading is dependent on the binding of Cdt1 to PCNA, which indicates that PCNA docking activates the pre-formed Cdt1-Cul4(DDB1) ligase complex.