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A431+CAL A/OKA ACID (2HR) Lysate

Name: A431+CAL A/OKA ACID (2HR) Lysate
SKU: ABIN968887
Availability: InStock

WB Whole Cell Lysate Cell Lysate A431 Cells A431 cells were grown to 80-90% confluency, serum starved overnight, then treated with 10 nM calyculin A and 500 nM okadaic acid for 2 hours at 37°C.

Produktnummer ABIN968887

592,37 €

Zzgl. Versandkosten 20,00 € und MwSt

500 μg

Lieferung nach: Deutschland

Lieferung in 12 bis 15 Werktagen

Kurzübersicht für A431+CAL A/OKA ACID (2HR) Lysate (ABIN968887)

Applikation

Western Blotting (WB)

Lysed Cells

A431 Cells

Lysate Type

Cell Lysate

Lysate Fraction

Whole Cell Lysate

Lysate Treatment

A431 cells were grown to 80-90% confluency, serum starved overnight, then treated with 10 nM calyculin A and 500 nM okadaic acid for 2 hours at 37°C.

Lysatspezies

Human Cells

Produktmerkmale

BD Transduction Laboratories™ A431 + Calyculin A/Okadaic Acid Cell Lysate

Aufreinigung

Purified
NLRP3 293T Cell Transient Overexpression Lysate(Denatured)
NLRP3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

TNFRSF13C 293T Cell Transient Overexpression Lysate(Denatured)
TNFRSF13C WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

ZMYND19 293T Cell Transient Overexpression Lysate(Denatured)
ZMYND19 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

MRGPRX2 293T Cell Transient Overexpression Lysate(Denatured)
MRGPRX2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

DDIT4L 293T Cell Transient Overexpression Lysate(Denatured)
DDIT4L WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

NXNL1 293T Cell Transient Overexpression Lysate(Denatured)
NXNL1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

LOC124220 293T Cell Transient Overexpression Lysate(Denatured)
ZG16B WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

TMEM42 293T Cell Transient Overexpression Lysate(Denatured)
TMEM42 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

SIRPD 293T Cell Transient Overexpression Lysate(Denatured)
SIRPD WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

RASGRP4 293T Cell Transient Overexpression Lysate(Denatured)
RASGRP4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

Applikationshinweise

Optimal working dilution should be determined by the investigator.

Kommentare

Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Protokoll

Cell or tissue lysates are a useful control for the western blotting application. A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel. A431 cells were grown to 80-90 % confluency, serum starved overnight, then treated with 10 nM calyculin A and 500 nM okadaic acid for 2 hours at 37 °C.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1.0 mg/mL

Buffer

SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)

Lagerung

-20 °C

Informationen zur Lagerung

Do not dilute or boil. Store undiluted at -20° C.