Maus Embryonic Fibroblast Whole Cell Lysate
Kurzübersicht für Maus Embryonic Fibroblast Whole Cell Lysate (ABIN964051)
Proteinspezies
Applikation
Lysed Cells
Lysate Type
Lysate Fraction
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Lysatspezies
- Maus
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Hersteller Produkt- Nr.
- w10-001-371
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Hersteller
- Rockland
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Spezifität
- The cells were grown in RPMI supplemented with 10% FBS (Fetal Bovine Serum). The lysate was prepared by first washing the cells in PBS. Washed cells are then incubated on ice in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris Cl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.25% sodium deoxycholic acid to lyse the cells. Protein integrity is ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate and 1 µM Pepstatin A). Phosphatase inhibitors include 1 mM Sodium Fluoride and 1mM Na3VO4. Cell debris was removed by membrane filtration. Protein concentration was determined by Lowry assay using a commercially available kit. The protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.
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Produktmerkmale
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Cell Line: Mouse Embryonic Fibroblast
Induction: None (Control) -
Sterilität
- Sterile filtered
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MEIS1 293T Cell Transient Overexpression Lysate(Denatured)
MEIS1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
SLC26A2 293T Cell Transient Overexpression Lysate(Denatured)SLC26A2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PTGS2 293T Cell Transient Overexpression Lysate(Denatured)PTGS2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PTGER2 293T Cell Transient Overexpression Lysate(Denatured)PTGER2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PTGS1 293T Cell Transient Overexpression Lysate(Denatured)PTGS1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
LSAMP 293T Cell Transient Overexpression Lysate(Denatured)LSAMP WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
MICB 293T Cell Transient Overexpression Lysate(Denatured)MICB WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
HRG 293T Cell Transient Overexpression Lysate(Denatured)HRG WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
HOXB5 293T Cell Transient Overexpression Lysate(Denatured)HOXB5 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
MGMT 293T Cell Transient Overexpression Lysate(Denatured)MGMT WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
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Applikationshinweise
- Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.
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Kommentare
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Lysate Fractionation: Whole Cell Lysate
Lysate Stimulation: Not Stimulated
Lysate Tissue Culture: Tissue Culture -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1.0 mg/mL
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Buffer
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
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Handhabung
- Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 °C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.
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Lagerung
- -80 °C
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Haltbarkeit
- 3 months
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Hintergrund
- Ready-to-use whole cell lysates produced are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
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