Madin-Darby bovine kidney (MDBK) cells Lysate
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- Applikation
- Western Blotting (WB)
- Produktmerkmale
- Madin-Darby Bovine Kidney (MDBK) Cell Lysate,Total cell Iysates are useful as both positive and negative controls in immunoblotting. A431, a human epidermoid carcinoma cell line, and the EGF-stimulated A431 Iysates are used as negative and positive controls, respectively, when studying the phosphorylation cascade initiated by ligand binding to receptor tyrosine kinases. Both A431 and normal human fibroblasts are valuable positive controls for antibodies to proteins expressed in these cells. The mouse fibroblast cell line 3T3 and its RSV-transformed counterpart serve as negative and positive controls, respectively, for antibodies to proteins that are phosphorylated as a result of transformation by RSV (Rous sarcoma virus). Madin-Darby bovine kidney (MDBK) cell Iysate displays proteins expressed in bovine kidney and PC12 (rat phaeochromocytoma) cell Iysate can be expected to mirror the protein expression of cells and tissues of neuroectodermal origin. Likewise, Jurkat cells express the proteins unique to human T-cells. Rat brain is a widely used positive control for those proteins that are exclusively expressed in brain. At right is an example of the use of positive and negative control Iysates in western blotting. The blot of 3T3 cell Iysate (lane 1) and RSV-transformed 3T3 (lane 2) was probed with anti-phosphotyrosine peroxidase conjugate to reveal those proteins that are tyrosine-phosphorylated in response to transformation by
- Lysate Type
- Cell Lysate
- Lysate Fraction
- Whole Cell Lysate
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IL25 293T Cell Transient Overexpression Lysate(Denatured)
IL25 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PHKG2 293T Cell Transient Overexpression Lysate(Denatured)PHKG2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PIK3R1 293T Cell Transient Overexpression Lysate(Denatured)PI3K p85a WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PLK1 293T Cell Transient Overexpression Lysate(Denatured)PLK1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PLOD2 293T Cell Transient Overexpression Lysate(Denatured)PLOD2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PLSCR1 293T Cell Transient Overexpression Lysate(Denatured)PLSCR1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PLCG2 293T Cell Transient Overexpression Lysate(Denatured)PLCG2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PLTP 293T Cell Transient Overexpression Lysate(Denatured)PLTP WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
POU4F3 293T Cell Transient Overexpression Lysate(Denatured)POU4F3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
PPARA 293T Cell Transient Overexpression Lysate(Denatured)PPARA WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
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- Applikationshinweise
- Suggested use: A volume of 10-25 µL is suggested for immunobloting.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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