HeLa whole-cell extract (TNF-α stimulated)
Kurzübersicht für HeLa whole-cell extract (TNF-α stimulated) (ABIN2669814)
Lysed Cells
Lysate Type
Lysate Fraction
Lysate Treatment
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Lysatspezies
- Human Cells
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Produktmerkmale
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Origin: Human Cervix Adenocarcinoma
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Bestandteile
- 2 x 125 μg of HeLa whole-cell extract (TNF-α stimulated) at 2.50 μg/μl
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NLRP3 293T Cell Transient Overexpression Lysate(Denatured)
NLRP3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
TNFRSF13C 293T Cell Transient Overexpression Lysate(Denatured)TNFRSF13C WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
ZMYND19 293T Cell Transient Overexpression Lysate(Denatured)ZMYND19 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
MRGPRX2 293T Cell Transient Overexpression Lysate(Denatured)MRGPRX2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
DDIT4L 293T Cell Transient Overexpression Lysate(Denatured)DDIT4L WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
NXNL1 293T Cell Transient Overexpression Lysate(Denatured)NXNL1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
LOC124220 293T Cell Transient Overexpression Lysate(Denatured)ZG16B WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
TMEM42 293T Cell Transient Overexpression Lysate(Denatured)TMEM42 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
SIRPD 293T Cell Transient Overexpression Lysate(Denatured)SIRPD WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
RASGRP4 293T Cell Transient Overexpression Lysate(Denatured)RASGRP4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 2.50 μg/μL
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Buffer
- The HeLa whole-cell extracts have been collected in Lysis Buffer after a 30-minute incubation with or without TNF-α (20 ng/mL). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20 % glycerol, 1 % Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA. The protein content has been determined by a Bradford-based assay.
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Handhabung
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
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Lagerung
- -80 °C
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Informationen zur Lagerung
- To ensure stability, extracts should be stored at -80°C.
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