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HeLa whole-cell extract (TNF-α stimulated)

Whole Cell Lysate Cell Lysate HeLa Cells TNF-α stimulated
Produktnummer ABIN2669814
  • Lysatspezies
    Human Cells
    Produktmerkmale

    Origin: Human Cervix Adenocarcinoma

    Bestandteile
    2 x 125 μg of HeLa whole-cell extract (TNF-α stimulated) at 2.50 μg/μl
    Lysed Cells
    HeLa Cells
    Lysate Type
    Cell Lysate
    Lysate Fraction
    Whole Cell Lysate
    Lysate Treatment
    TNF-α stimulated
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    2.50 μg/μL
    Buffer
    The HeLa whole-cell extracts have been collected in Lysis Buffer after a 30-minute incubation with or without TNF-α (20 ng/mL). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20 % glycerol, 1 % Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA. The protein content has been determined by a Bradford-based assay.
    Handhabung
    It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    To ensure stability, extracts should be stored at -80°C.
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