Jurkat nuclear extract (TPA + CI stimulated)
Kurzübersicht für Jurkat nuclear extract (TPA + CI stimulated) (ABIN2669798)
Lysed Cells
Lysate Type
Lysate Fraction
Lysate Treatment
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Lysatspezies
- Human Cells
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Produktmerkmale
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Origin: Human T lymphoblast, acute T cell leukemia
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Bestandteile
- 2 x 100 μg of Jurkat nuclear extract (TPA + CI stimulated) at 2.5 μg/μl
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NLRP3 293T Cell Transient Overexpression Lysate(Denatured)
NLRP3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
TNFRSF13C 293T Cell Transient Overexpression Lysate(Denatured)TNFRSF13C WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
ZMYND19 293T Cell Transient Overexpression Lysate(Denatured)ZMYND19 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
MRGPRX2 293T Cell Transient Overexpression Lysate(Denatured)MRGPRX2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
DDIT4L 293T Cell Transient Overexpression Lysate(Denatured)DDIT4L WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
NXNL1 293T Cell Transient Overexpression Lysate(Denatured)NXNL1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
LOC124220 293T Cell Transient Overexpression Lysate(Denatured)ZG16B WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
TMEM42 293T Cell Transient Overexpression Lysate(Denatured)TMEM42 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
SIRPD 293T Cell Transient Overexpression Lysate(Denatured)SIRPD WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
RASGRP4 293T Cell Transient Overexpression Lysate(Denatured)RASGRP4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 2.5 μg/μL
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Buffer
- Nuclear extracts are supplied in dilution buffer (20 mM Hepes ( pH 7.9), 100 mM KCl, 1 mM MgCl2, 20 % glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 μM calcium ionophore A23187 (CI) for two hours at 37 °C immediately prior to harvesting.
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Konservierungsmittel
- Dithiothreitol (DTT)
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Vorsichtsmaßnahmen
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
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Lagerung
- -80 °C
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Informationen zur Lagerung
- To ensure stability, extracts should be stored at -80°C.
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