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Hippocampus (Alzheimer's Disease) Lysate

WB Whole Tissue Lysate Tissue Lysate
Produktnummer ABIN2456590
  • Lysatspezies
    Human
    Applikation
    Western Blotting (WB)
    Produktmerkmale
    Human hippocamps tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The human hippocamps tissue total protein is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the hippocamps tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The hippocamps tissue is then Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.

    Source: Human Hippocampus tissue
    Diagnosis: Diseased
    Tissue or Cell type: Brain: Hippocampus
    Lysate Type
    Tissue Lysate
    Lysate Fraction
    Whole Tissue Lysate
  • Applikationshinweise
    Hippocampus lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    2 mg/mL
    Buffer
    HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
    Handhabung
    Repeated freezing and thawing is not recommended.
    Lagerung
    4 °C/-80 °C
    Informationen zur Lagerung
    Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
    Haltbarkeit
    6 months
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