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Lhcb4 Antikörper

Der Kaninchen Polyklonal Anti-Lhcb4-Antikörper wurde für WB validiert. Er ist geeignet, Lhcb4 in Proben von Arabidopsis thaliana, Green bean, Oryza sativa, Pisum sativum und Mais (Zea mays) zu detektieren. Es ist 1 Publikation verfügbar.
Produktnummer ABIN5684016

Kurzübersicht für Lhcb4 Antikörper (ABIN5684016)

Target

Lhcb4 (Cp29 Chlorophyll A/B Binding Protein of Plant Psii (Lhcb4))

Reaktivität

Arabidopsis thaliana, Green bean, Oryza sativa, Pisum sativum, Mais (Zea mays)

Wirt

Kaninchen

Klonalität

Polyklonal

Applikation

Western Blotting (WB)
  • Spezifität

    The antibody is very specific for salmonid inducible form - hsp70.

    Kreuzreaktivität (Details)

    No cross-reactivity with: Chlamydomonas reinhardtii

    Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 31.9 / 29 kDa for Arabidopsis thaliana

    Aufreinigung

    affinity purified

    Immunogen

    BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At5g08940).

    Isotyp

    IgG
  • Applikationshinweise

    Recommended Dilution: 1 : 2000 - 1 : 5000 with standard ECL (WB).

    Kommentare

    for image of detection please refer to Kusaba et al. (2007)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 200 µL of sterile water

    Buffer

    PBS pH 7.4

    Handhabung

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Lagerung

    -20 °C
  • Yamatani, Sato, Masuda, Kato, Morita, Fukunaga, Nagamura, Nishimura, Sakamoto, Tanaka, Kusaba: "NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - protein complexes during leaf senescence." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 652-62, (2013) (PubMed).

  • Target

    Lhcb4 (Cp29 Chlorophyll A/B Binding Protein of Plant Psii (Lhcb4))

    Hintergrund

    AGI Code: At5g01530
    Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with Photosystem II in plants and algae. Lhcb4 has been suggested to act in the regulation of the chl a excited state concentration of PSII because of its ability of sensing lumenal pH resulting in reversible phosphorylation. In Arabidopsis thaliana 2 genes code for two isoforms Lhcb4.1 and Lhcb4.2. A third isoform (Lhcb4.3, At5g40100), probably only present in dicots, has found to be differently regulated and therefore has been denoted as Lhcb8.

    Molekulargewicht

    expected: 31.9 kDa, apparent: 29 kDa for Arabidopsis thaliana

    UniProt

    Q9XF88, Q07473
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