Aflatoxin M1 ELISA Kit

Produktnummer ABIN997085

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Aspergillus Aflatoxin M1.

Kurzübersicht für Aflatoxin M1 ELISA Kit (ABIN997085)

Target: Aflatoxin M1

Reaktivität: Aspergillus

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Produktdetails Aflatoxin M1 ELISA Kit

Verwendungszweck: Aflatoxin M1 quantitative test is based on the principle of the enzyme linked immunosorbent assay.

Analytische Methode: Quantitative

Sensitivität: < 10 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

Because the standards are concentrated 10x, they have to be diluted by the enclosed standard/sample diluent 1:10 (e.g. 50 μL standard + 450 μL diluent), before using them in the assay procedure.

Testdurchführung:

1. Prepare samples as described above.
   2. Pipet 100 μL diluted (1:10) standards or prepared samples in duplicate into the appropriate wells of the microtiter plate. Immediately add 50 μL aflatoxin M1 antibody into each well.
   3. Cover the microtiter plate with a plastic foil and incubate for 60 minutes at room temperature on a microtiter plate shaker (or 90 minutes without shaker).
   4. Wash the plate three times as follows: Discard the contents of the wells (dump or aspirate). Pipet 300 μL of diluted washing solution into each well. After the third repetition empty the wells again and remove residual liquid by striking the plate against a paper towel. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbencies.
   5. Pipet 100 μL of conjugate (anti-rabbit-IgG-HRP) into each well.
   6. Cover the microtiter plate with a plastic foil and incubate for 60 minutes at room temperature on a microtiter plate shaker (or 90 minutes without shaker).
   7. Wash the plate as outlined in 4.
   8. Pipet 100 μL of substrate solution into each well.
   9. Allow the reaction to develop in the dark (e.g. cupboard or drawer, the chromogen is light-sensitive) for 20 minutes at room temperature.
   10. Stop enzyme reaction by adding 100 μL of stop solution (0.5 M H2SO4) into each well. The blue colour will turn yellow upon addition.
   11. After thorough mixing, measure absorbance at 450 nm (reference wavelength 620 nm), using an ELISA reader. The colour is stable for 30 minutes.

Ergebnisberechnung:

4. The diluted samples must be further converted by the appropriate dilution factor (50 for the above described extraction). The factor is dependent on the sample preparation procedure employed. TYPICAL STANDARD VALUES The following table contains an example for a typical standard curve. The binding is calculated as percent of the absorption of the 0 pg/mL standard. These values are only an example and should not be used instead of the standard curve which has to be measured in every new test. Aflotoxin B1 (pg/mL) ( % binding of 0 ng/mL) 0 100 10 90 40 85 100 70 400 40 1000 25 PERFORMANCE
   

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C

Details zu Aflatoxin M1

Target: Aflatoxin M1