Cryptosporidium ELISA Kit

Produktnummer ABIN997059

Das Cryptosporidium parvum Cryptosporidium ELISA Kit (ABIN997059) ist ein Colorimetric ELISA Kit zur Detektion von Cryptosporidium parvum Cryptosporidium. Das Kit wurde in 1 Publikation zitiert.

Kurzübersicht für Cryptosporidium ELISA Kit (ABIN997059)

Target: Cryptosporidium

Reaktivität: Cryptosporidium parvum

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Produktdetails

Verwendungszweck: This ELISA is an in vitro immunoassay for the qualitative determination of Cryptosporidium antigen in feces.

Analytische Methode: Qualitative

Spezifität: 100 %

Anwendungsinformationen

Probenmenge: 1 g

Testdauer: 1 - 2 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Referenzen

Nassar, Oyekale, Oluremi: "Prevalence of Cryptosporidium infection and related risk factors in children in Awo and Iragberi, Nigeria." in: Journal of immunoassay & immunochemistry, (2016) (PubMed).

Antigendetails

Target: Cryptosporidium

Substanzklasse: Parasite

Hintergrund: Cryptosporidium is a coccidian parasite that is recognized as an important enteric pathogen. The organism causes an acute, though self-limiting infection in immunocompetent individuals. Incubation periods of 1 to 12 days have been reported with most oocyst shedding ending by day 21. Symptoms range from mild to severe diarrhea with a variety of complications. The infection in immunocompromised patients is much more severe and may often be life threatening. Passage of fluid, up to 12 liters per day, has been reported. Multiple pathways of Cryptosporidium transmission have been implicated. These include animal to human, water contamination and person-to-person. The latter may include contact between members of the same household, day care centers, and homosexual men.

Diagnosis of Cryptosporidium infections was done originally by direct detection techniques. Of these, microscopic examination of stools using stains or fluorescence labeled antibodies has been the most common. However, this method relies on an experienced technician and subsequent observation of intact organisms. Because of the historically low proficiency of correct microscopic examinations, alternative diagnostic methods have been investigated. One important alternative has been the development of an antigen capture enzyme linked immunosorbent assay (ELISA) for use with stools. These tests, which have shown comparable sensitivity to experienced microscopic examinations, are fairly simple to perform and do not require the observation of intact organisms.