anti Trichinella IgG ELISA Kit

Produktnummer ABIN997050

Produktdetails

Target: anti Trichinella IgG

Reaktivität: Trichinella spiralis

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: The Trichinella ELISA Test is a qualitative enzyme immunoassay for the detection of antibodies to Trichinella, in samples of human serum or plasma.

Proben: Serum

Analytische Methode: Qualitative

Spezifität: 100 %

Benötigtes Material: 1. Pipettes
2. Squeeze bottle for washing strips (narrow tip is recommended)
3. Reagent grade water and graduated cylinder
4. Tubes for sample dilution
5. Absorbent paper
6. ELISA plate reader with a 450 nm and a 620-650 nm filter (optional if results are read visually).

Anwendungsinformationen

Kommentare:

Quality Control:
The use of controls allows validation of kit stability. The kit should not be used if any of the controls are out of range. Expected values for the controls are: Negative - 0.0 to 0.3 OD units Positive - 0.5 OD units and above
Limitations of procedure: Serologic results are an aid in diagnosis but cannot be used as the sole method of diagnosis. DAI CODE #35 Page 2 of 2

Probenmenge: 5 μL

Testdauer: 1 h

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

Preparation Wash Buffer - Remove cap and add contents of bottle to 475 mL of reagent grade water. Place diluted wash buffer into a squeeze bottle with a narrow tip opening.
Note:
Washings consist of filling to the top of each well, shaking out the contents and refilling. Avoid generating bubbles in the wells during the washing steps.

Aufbereitung der Proben:

Serological specimens should be collected under aseptic conditions. Coagulate blood and remove serum. Hemolysis is avoided through prompt separation of the serum from the clot. Serum should be stored at 2 - 8 °C if it is to be analyzed within a few days. Serum may be held for 3 to 6 months by storage at -20 °C or lower. Lipemic and strongly hemolytic serum should be avoided. Do not heat inactivate serum and avoid repeated freezing and thawing of samples. Test samples: Make a 1:64 dilution of patient's sera using the dilution buffer (e.g. 5 μL sera and 315 μL dilution buffer).

Testdurchführung:

1. Break off number of wells needed (two for controls plus number of samples) and place in strip holder.
   2. Add 100 μL (or two drops) of the negative control to well #1, 100 μL of the positive control to well #2 and 100 μL of the diluted (1:64) test samples to the remaining wells. Note: Negative and positive controls are supplied prediluted. Do not dilute further.
   3. Incubate at room temperature (15 to
   2. °C) for 10 minutes.
   4. Shake out contents and wash 3 times with the diluted wash buffer.
   5. Add 100 μL of the Enzyme Conjugate to each well. cc
   6. Incubate at room temperature for 5 minutes.
   7. Shake out contents and wash 3 times with wash buffer. Slap wells against paper towels to remove all of the wash buffer.
   8. Add 100 μL of the Chromogen to every well.
   9. Incubate at room temperature for 5 minutes.
   10. Add 100 μ1 of the Stop Solution and mix by tapping strip holder.

Ergebnisberechnung:

Visually: Look at each well against a white background (e.g. paper towel) and record as clear or +, ++ or +++ reaction. ELISA Reader: Zero reader on air. Set for bichromatic readings at 450/650-620 nm. Troubleshooting Negative control has excessive color after development. Reason: inadequate washings. Correction: wash more vigorously. Remove excessive liquid from the wells by tapping against an absorbent towel. Do not allow test wells to dry out. Interpretation of the Test Zero ELISA reader on air. Read all wells at 450/650 to 620 nm. Positive - Absorbance reading greater than 0.3 OD units. Negative - Absorbance reading less than 0.3 OD units. A negative OD reading indicates that the patient has no detectable level of antibodies. This may be due to lack of infection or poor immune response by the patient. Interpretation of Results -Visual Compare results to the controls. A sample should be interpreted as positive if the degree of color development is obvious and significant.
Date Adopted
The number of individuals showing positive results can vary significantly between populations and geographic regions. If possible, each laboratory should establish an expected range for its patient population.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Antigendetails

Target: anti Trichinella IgG

Substanzklasse: Antibody

Hintergrund: Trichinosis, the infection caused by the nematode Trichinella spiralis, is acquired by ingestion of raw or undercooked meats (primarily pork). Although the nematode may be found in a wide variety of animals worldwide, the domestic pig is the primary source of infection in developed nations. Serology has also been an important tool in the diagnosis of trichinosis for several decades. Various methodologies, such as ELISA, latex agglutination (LA), indirect hemagglutination (IHA) and bentonite flocculation (BFT) have been used. Although various classes of antibodies have been detected, no single class has shown superior diagnostic ability over the others. BFT has been the method of choice for serology but suffers from nonspecific reactions, some lack of sensitivity (measurable antibodies often do not appear until 3 to 4 weeks after infection) and difficulty in performing the test. Recently, an excretory-secretory (ES) antigen has been purified from the larvae of infected pigs. This antigen has a high degree of specificity for T. spiralis and has been used in several large scale studies.