HBcAb-IgM ELISA Kit

Produktnummer ABIN997018

Hepatitis B Virus (HBV) HBcAb-IgM ELISA-Kit Colorimetric-Assay zur Quantifizierung von Hepatitis B Virus (HBV) HBcAb-IgM.

Kurzübersicht für HBcAb-IgM ELISA Kit (ABIN997018)

Target: HBcAb-IgM (Anti-Hepatitis B Virus Core Antibody IgM (HBcAb-IgM))

Reaktivität: Hepatitis B Virus (HBV)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Serum

Produktdetails HBcAb-IgM ELISA Kit

Verwendungszweck: The HBcAb IgM ELISA is an enzyme-linked immunosorbent assay for the qualitative identification of IgM class antibodies to hepatitis B core antigen in human serum/plasma.

Analytische Methode: Qualitative

Spezifität: 99.3%

Sensitivität: 98.4%

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 1 - 2 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12-18 months

Details zu HBcAb-IgM

Target: HBcAb-IgM (Anti-Hepatitis B Virus Core Antibody IgM (HBcAb-IgM))

Andere Bezeichnung: HbcAb IgM

Substanzklasse: Antibody, Antibody

Hintergrund: Hepatitis B virus (HBV) is an enveloped, double-stranded DNA virus belonging to the Hepadnaviridae family and is recognized as the major cause of blood transmitted hepatitis together with hepatitis C virus (HCV). Infection with HBV induces a spectrum of clinical manifestations ranging from mild, inapparent disease to fulminant hepatitis, severe chronic liver diseases, which in some cases can lead to cirrhosis and carcinoma of the liver. Classification of a hepatitis B infection requires the identification of a number of serological markers expressed during three phases (incubation, acute and convalescent) of the infection. Now several diagnostic test are used for screening, clinical diagnosis and management of the disease. Hepatitis B “core ” antigen (HBcAg) is a major component of the viral structure.

HBcAg is composed of a single polypeptide of about 17 kD that is released upon disaggregation of the core particles, the antigen contains at least one immunological determinant. Antibodies to HBcAg (anti-HBc total antibody and IgM) appear shortly after the appearance of HBsAg and persist for life both in persons who have recovered from a hepatitis B infection and in those who develop HBsAg-carrier status but in rare cases, an HBV infection can also run its course without the appearance of immunologically detectable anti-HBc (usually in immunosuppressed patients). In chronic hepatitis, however, spikes of anti-HBc IgM synthesis are present, confirming reactivation of HBV in hepatocites and giving origin to permanent IgM low titers. Presence of IgM and total anti-HBc indicates an ongoing or recent HBV infection. When used in conjunction with tests for other HBV serological markers, a laboratory diagnosis or a rule out of HBV infection can be achieved.

This kit is a two-step incubation, solid phase antibody capture ELISA assay, in which polystyrene microwell strips are pre-coated with antibodies directed to human immunoglobulin M proteins (anti-µ chain). The patient’s serum/plasma sample is added and during the first incubation step, any IgM-class antibodies will be captured inside the wells. After washing out all other components of the sample and in particular IgG-class antibodies, the specific anti-HBc IgM captured on the solid phase is detected by the addition of purified HBcAg, labeled with anti-HBc monoclonal antibody conjugated to horseradish peroxidase (HRP-Conjugate). During the second incubation, the HRP-conjugated antigens will specifically react only with anti-HBc IgM antibodies, and after washing to remove the unbound HRP- conjugate, Chromogen solutions are added to the wells. In presence of the (anti-µ chain)-(anti-HBc IgM)-(HBcAg-Ab (HRP)) immunocomplex, the colorless Chromogens are hydrolyzed by the bound HRP-conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for anti-HBc IgM remain colorless.