PTH ELISA Kit

Produktnummer ABIN996928

Chemical PTH ELISA Kit, Colorimetric Assay für die Quantifizierung von Chemical PTH.

Kurzübersicht für PTH ELISA Kit (ABIN996928)

Target: PTH (Parathyroid Hormone (PTH))

Reaktivität: Chemical

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Serum

Produktdetails PTH ELISA Kit

Verwendungszweck: Intact-PTH ELISA is intended for the quantitative determination of IntactPTH (Parathyroid Hormone) in human serum.

Analytische Methode: Quantitative

Spezifität: 100 %

Sensitivität: 1.72 pg/mL

Anwendungsinformationen

Probenmenge: 25 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu PTH

Target: PTH (Parathyroid Hormone (PTH))

Hintergrund: PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a pre-proparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25-amino acid sequence, preproparathyroid hormone is converted to an intermediate, a 90-amino acid polypeptide, proparathyroid hormone. With additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an 84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and mid-region fragments. In subjects with renal insufficiency, C-terminal and mid-region PTH assays typically give elevated PTH results, as reflected by impaired renal clearance.

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process