ENO2/NSE ELISA Kit

Produktnummer ABIN996903

Das ENO2/NSE ELISA Kit (ABIN996903) ist ein Colorimetric ELISA Kit zur Detektion von ENO2/NSE.

Kurzübersicht für ENO2/NSE ELISA Kit (ABIN996903)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0-200 ng/mL

Applikation: ELISA

Proben: Serum

Produktdetails ENO2/NSE ELISA Kit

Untere Nachweisgrenze: 0 ng/mL

Verwendungszweck: The Neuron-Specific Enolase (NSE) enzyme linked immunosorbent assay (ELISA) provides quantitative measurement of human NSE in serum to aid in the clinical evaluation of patients suspected of having small cell lung cancer, and other related diseases.

Analytische Methode: Quantitative

Spezifität: 98.7 %

Sensitivität: 15 ng/mL

Anwendungsinformationen

Probenmenge: 15 μL

Testdauer: 1 - 2 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12-14 months

Details zu ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Andere Bezeichnung: NSE

Hintergrund: The glycolytic enzyme enolase (2-phosph-D-glycerate hydrolyase) exists as several dimeric isoenzymes ( alphaalpha , alphabeta alphagamma and gammagamma ) composed of three distinct subunits, alpha , beta , and gamma . Three isoenzymes are found in human brain: alphaalpha , alphagamma and gammagamma . The alphagamma and gammagamma -enolase isoenzymes are also known as neuron-specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuronendocrine cells. The NSE levels are low in health and benign subjects. Elevated levels are commonly found in patients with malignant tumors with neuronendocrine differentiation, especially small cell lung cancer and neuroblastoma. Lung cancer is one of the most spread cancer forms with incidences about 50~100 per 100,000 population.
Approximately 20% of the lung cancer is small cell lung cancer. Patients with small cell lung cancer show various proportions of alphagamma and gammagamma isoenzyme. The determination of NSE should detect and gamma isoforms with the same sensitivity. The antibodies for this particular assay are specific for the gamma -subunit without cross reactivity with alpha or beta subunits. NSE Kits are reported to be useful diagnostic marker for lung cancer, neuroblastoma, and melanoma, seminoma (S) and in injury of central nervous system. In addition to the above, NSE can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer.