Urinary Diacetylspermine ELISA Kit

Produktnummer ABIN956345

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Nagetiere Urinary Diacetylspermine.

Kurzübersicht für Urinary Diacetylspermine ELISA Kit (ABIN956345)

Target: Urinary Diacetylspermine

Reaktivität: Nagetiere

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Proben: Urine

Produktdetails

Verwendungszweck: The Urinary Diacetylspermine EIA is for the quantitative determination of urinary diacetylspermine in rodents.

Analytische Methode: Quantitative

Produktmerkmale: Our kit is convenient for use quantifying the amount of urinary diacetylspermine by using the ELSA method. This kit is for research use only. Highly sensitive and specific Strip type well, antigen pre-coated microplate. Assay range: 6.25-200 nM

Bestandteile: Wash Solution: 30 mL 20x concentrate
Antibody Diluent: 20 mL Antibody
Plate: 96 wells
Calibrator: 2 x 250 µL Anti-Diacetylspermine
Antibody: 60 µL 100x concentrate
HRP-Anti Rabbit IgG Antibody: 80 µL 80x concentrate
OPD (o-phenylendiamine) tablets x2
Substrate Solution: 30 mL
Stop Solution: 15 mL
Dilution Plate: 1 plate.

Benötigtes Material: Micropipette and tip
Plate washer
Plate reader

Anwendungsinformationen

Plattentyp: Pre-coated

Aufbereitung der Proben:

1. Collect urine in sampling tube. Add 0.1% Na2N3 at final concentration.
   2. After centrifugation at 1500 rpm for 5 minutes, dilute the resulting supernatant over 4 times with distilled water.
   3. Measure the amount of creatinine in the remaining diluted supernatant for compensation.
   4. Prepared urine should be kept below -30°C if necessary.
   
   Procedure: Pre-reaction: Prepare the calibrator control wells containing 70 µL of anti-Diacetylspermine antibody solution and 70 µL of the 6 calibrators (200, 100, 50, 25, 12.5, 6.25 nM) in the dilution plate. Likewise prepare the experimental wells containing 70 µL of anti-Diacetylspermine antibody solution and 70 µL of prepared urinary sample in the same plate. After settlement, incubate at room temperature for 1 hour.

Ergebnisberechnung:

In general, the concentration of urine changes easily by the amount of water taken in or by the environment. The amount of urinary creatinine depends on the amount of muscle and their measurements correlate positively.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: All reagents should be stored at -30°C.

Antigendetails

Target: Urinary Diacetylspermine

Hintergrund: Polyamines are generally believed to function both in protein synthesis and DNA synthesis leading to control cell proliferation. It is reported that the total amount of urinary polyamines are elevated in cancer patients. Assays have already been developed to measure the total amount of these urinary polyamines. Recently two diacetyl-derivatives, N1, N12-diacetylspermine and N1, N8-diacetylspermine, were found to correlate to the status of disease more closely than the total amount of polyamines.