ACE Inhibition Screening Kit

Produktnummer ABIN956241

Kurzübersicht für ACE Inhibition Screening Kit (ABIN956241)

Target: ACE Inhibition Screening

Applikation: Screening Assay (ScA)

Produktdetails

Bestandteile: Substrate Buffer, 2 vials
Enzyme A, 2 vials
Enzyme B, 2 vials
Enzyme C, 2 vials
Coenzyme, 2 vials
Indicator solution, 2 vials.

Benötigtes Material: Microplate reader (450 nm filter).
96-well microplate.
2-20 µL & 20-200 µL pipettes and a multi-channel pipette.
Incubator.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C. This kit is stable at 4°C for six months in unopened condition. Use up enzyme working solution and indicator working solution immediately. Do not freeze this kit.

Haltbarkeit: 6 months

Antigendetails

Target: ACE Inhibition Screening

Hintergrund: Angiotensin-converting enzyme (ACE) is one of the vasopressor principle. ACE converts angiotensin I to angiotensin II, that has a vasopressor action, in the renin-angiotensin system and also inactivates bradykinin that is an antihypertensive peptide. Recently, modification of the conventional ACE inhibition assay procedure has been requested because of the use of harmful organic solvent such as ethyl acetate for the extraction of hippuric acid cleaved from Hippuryl-His-Leu by ACE and its complicated procedure.The ACE Inhibition Screening Kit is a simple and convenient kit to measure the ACE inhibitory activity. 3- Hydroxybutyryl-Gly-Gly-Gly (3HB-GGG) is utilized as a substrate for ACE, and the amount of cleaved 3-hydroxybutyric acid (3HB) from 3HB-GGG is measured by the enzymatic method. Neither organic solvent nor extraction procedure is required through the whole procedure. In addition, since the ACE Inhibition Screening Kit is optimized for 96- well microplate assay, a large number of samples can be tested at once.