Total Antioxidant Capacity ELISA Kit

Produktnummer ABIN956217

Produktdetails

Target: Total Antioxidant Capacity

Reaktivität: Chemical

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 21.9-4378 μmol/L

Untere Nachweisgrenze: 21.9 μmol/L

Applikation: ELISA

Analytische Methode: Quantitative

Sensitivität: 21.9 μmol/L

Produktmerkmale: Oxidative stress plays an important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging. Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. PAO can detect not only hydrophilic antioxidants such as Vitamin C and glutathione, but can also detect hydrophobic antioxidants such as Vitamin E. Applicable for the assessment of total antioxidants of serum, foods, and beverage samples. For research use only.Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine), and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed.

Bestandteile: 1. Calibrator (Uric acid powder): 1 vial (for 2 mM), Dissolve with distilled water.
2. Sample diluent: 60 mL x 1 bottle, Ready to use.
3. Cu++ solution: 5 mL x 1 bottle, Ready to use.
4. Stop solution: 5 mL x 1 bottle, Ready to use.
5. Microtiter plate: 1 plate

Benötigtes Material: A. A microplate reader (measuring wavelength 490 nm)
B. Pipettes and pipette chips
C. Plastic test tubes
D. Distilled water
E. NaOH, HCl solution and pH meter (Not required if calibrators are prepared with distilled water only.).

Anwendungsinformationen

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT

Informationen zur Lagerung: Store at room temperature until the expiration date listed on the kit box label.

Haltbarkeit: The expiry date is stated on the label.

Antigendetails

Target: Total Antioxidant Capacity

Andere Bezeichnung: Total Antioxidant Capacity (PAO)