Kurzübersicht für Total Antioxidant Capacity ELISA Kit (ABIN956217)
Target
Total Antioxidant Capacity
Reaktivität
Chemical
Nachweismethode
Colorimetric
Methodentyp
Competition ELISA
Detektionsbereich
21.9-4378 μmol/L
Applikation
ELISA
Untere Nachweisgrenze
21.9 μmol/L
Analytische Methode
Quantitative
Sensitivität
21.9 μmol/L
Produktmerkmale
Oxidative stress plays an important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging. Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. PAO can detect not only hydrophilic antioxidants such as Vitamin C and glutathione, but can also detect hydrophobic antioxidants such as Vitamin E. Applicable for the assessment of total antioxidants of serum, foods, and beverage samples. For research use only.Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine), and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed.
Bestandteile
1. Calibrator (Uric acid powder): 1 vial (for 2 mM), Dissolve with distilled water. 2. Sample diluent: 60 mL x 1 bottle, Ready to use. 3. Cu++ solution: 5 mL x 1 bottle, Ready to use. 4. Stop solution: 5 mL x 1 bottle, Ready to use. 5. Microtiter plate: 1 plate
Benötigtes Material
A. A microplate reader (measuring wavelength 490 nm) B. Pipettes and pipette chips C. Plastic test tubes D. Distilled water E. NaOH, HCl solution and pH meter (Not required if calibrators are prepared with distilled water only.).