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Total Antioxidant Capacity ELISA Kit

Dieses Chemical ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Chemical zu quantifizieren.
Produktnummer ABIN956217

Kurzübersicht für Total Antioxidant Capacity ELISA Kit (ABIN956217)

Target

Total Antioxidant Capacity

Reaktivität

Chemical

Nachweismethode

Colorimetric

Methodentyp

Competition ELISA

Detektionsbereich

21.9-4378 μmol/L

Applikation

ELISA
  • Untere Nachweisgrenze

    21.9 μmol/L

    Analytische Methode

    Quantitative

    Sensitivität

    21.9 μmol/L

    Produktmerkmale

    Oxidative stress plays an important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging. Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. PAO can detect not only hydrophilic antioxidants such as Vitamin C and glutathione, but can also detect hydrophobic antioxidants such as Vitamin E. Applicable for the assessment of total antioxidants of serum, foods, and beverage samples. For research use only.Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine), and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed.

    Bestandteile

    1. Calibrator (Uric acid powder): 1 vial (for 2 mM), Dissolve with distilled water.
    2. Sample diluent: 60 mL x 1 bottle, Ready to use.
    3. Cu++ solution: 5 mL x 1 bottle, Ready to use.
    4. Stop solution: 5 mL x 1 bottle, Ready to use.
    5. Microtiter plate: 1 plate

    Benötigtes Material

    A. A microplate reader (measuring wavelength 490 nm)
    B. Pipettes and pipette chips
    C. Plastic test tubes
    D. Distilled water
    E. NaOH, HCl solution and pH meter (Not required if calibrators are prepared with distilled water only.).
  • Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    RT

    Informationen zur Lagerung

    Store at room temperature until the expiration date listed on the kit box label.

    Haltbarkeit

    The expiry date is stated on the label.
  • Target

    Total Antioxidant Capacity

    Andere Bezeichnung

    Total Antioxidant Capacity (PAO)
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