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Glucuronidase beta ELISA Kit

GUSB Reaktivität: Human Colorimetric Sandwich ELISA 0.156-10 ng/mL
Produktnummer ABIN955874
  • Target Alle Glucuronidase beta (GUSB) ELISA Kits anzeigen
    Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))
    Reaktivität
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156-10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of human GUSb in serum, plasma and other biological fluids.
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of human GUSb. No significant cross-reactivity or interference between human GUSb and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between human GUSb and all the analogues, therefore, cross reaction may still exist.
    Sensitivität
    The minimum detectable dose of human GUSb is typically less than 0.074 ng/mL.
    The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.
    Produktmerkmale
    The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to GUSb. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GUSb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GUSb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of GUSb in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
    Bestandteile
    Pre-coated, ready to use 96-well strip plate (1x)
    Calibrator (lyophilized) (2x)
    Calibrator Diluent (1 x 20 mL)
    Detection Reagent A (1 x 120 µL)
    Detection Reagent B (1 x 120 µL)
    Assay Diluent A (2X concentrate) (1 x 6 mL)
    Assay Diluent B (2X concentrate) (1 x 6 mL)
    TMB Substrate (1 x 9 mL)
    Stop Solution (1 x 6 mL)
    Wash Buffer (30X concentrate) (1 x 20 mL)
    Plate sealer for 96 wells (4x).
    Benötigtes Material
    1. Microplate reader with 450 +/- 10 nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. De-ionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Kommentare

    The calibration curve concentrations used for the ELISA's were 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, 0.156 ng/mL.

    Plattentyp
    Pre-coated
    Ergebnisberechnung

    Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with GUSb concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
    Haltbarkeit
    The expiry date is stated on the label.
  • Target Alle Glucuronidase beta (GUSB) ELISA Kits anzeigen
    Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))
    Abstract
    GUSB Produkte
    Synonyme
    GUSB ELISA Kit, beta-GUS ELISA Kit, gus ELISA Kit, BG ELISA Kit, MPS7 ELISA Kit, AI747421 ELISA Kit, Gur ELISA Kit, Gus ELISA Kit, Gus-r ELISA Kit, Gus-s ELISA Kit, Gus-t ELISA Kit, Gus-u ELISA Kit, Gut ELISA Kit, asd ELISA Kit, g ELISA Kit, Ac2-223 ELISA Kit, si:ch211-160e1.7 ELISA Kit, si:ct573103.7 ELISA Kit, glucuronidase beta ELISA Kit, beta-D-glucuronidase ELISA Kit, beta-glucuronidase ELISA Kit, beta glucuronidase ELISA Kit, glucuronidase, beta ELISA Kit, GUSB ELISA Kit, bglR ELISA Kit, SSO_RS14735 ELISA Kit, Glu ELISA Kit, Gusb ELISA Kit, gusb ELISA Kit
    Pathways
    Glycosaminoglycan Metabolic Process
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