Lactoferrin ELISA Kit

Produktnummer ABIN930713

Produktdetails Lactoferrin ELISA Kit

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reaktivität: Säugetier

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Analytische Methode: Quantitative

Produktmerkmale: ELISA kit for the detection of Lactoferrin in the research laboratory
Alternative Names: Lactoferrin ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end-user

Plattentyp: Pre-coated

Testdurchführung:

Highly purified Lactoferrin is bound to microwells . Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Andere Bezeichnung: Lactoferrin (LTF Produkte)

Hintergrund: Lactoferrin (LF) is an iron-binding protein, which occurs in high concentrations in secretions at mucosa surfaces, in tears and in milk. LF also resides in the specific granules of polymorphnuclear neutrophil leukocytes (PMN) and becomes exocytosed upon PMN activation. During active inflammatory disease, raised serum levels of LF can be measured. The physiological anti microbial effect of Lactoferrin depends on its iron-binding capacity, because most bacteria require iron for their own physiological pathways. LF inhibits myelopoiesis, prevents complement activation and prevents the formation of hydroxl radicals. It is quite possible that LF has several important roles, like secretory IgA, as a nonspecific anti-phlogistic defence factor at mucosal surfaces. LF belongs to the pANCA, depending on the redistribuiton from the granules toward the nuclei, upon ethanol fixation. Autoantibodies against Lactoferrin occur in higher frequency in patients with rheumatoid vasculitis (RV), colitis ulcerosa (CU) and primary sclerosing cholangitis (PSC).
Synonyms: Lactoferrin ELISA kit.

Pathways: Transition Metal Ion Homeostasis