Elastase ELISA Kit

Produktnummer ABIN930710

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Säugetier Elastase.

Kurzübersicht für Elastase ELISA Kit (ABIN930710)

Target: Elastase

Reaktivität: Säugetier

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Produktdetails Elastase ELISA Kit

Analytische Methode: Quantitative

Produktmerkmale: ELISA kit for the detection of Elastase in the research laboratory
Alternative Names: Elastase ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end-user

Plattentyp: Pre-coated

Testdurchführung:

Highly purified Elastase is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu Elastase

Target: Elastase

Hintergrund: Elastase is a serine protease with a sequence homology of 54 % to that of proteinase 3. It occurs mainly in polymorphnuclear neutrophilic granulocytes (PMN), in macrophages and endothelial cells. The dismantling of proteoglycans by neutrophiles is mainly due to elastase' proteolytic activity. Furthermore, elastase participates decisively in tissue destruction connected with emphysemas and rheumatoid arthritis. Autoantibodies against this antigen are generally associated with inflammatory rheumatic disorders, e.g. rheumatoid arthritis and vasculitis.
Synonyms: Elastase ELISA kit.