Anti-Cardiolipin I IgA/IgG/IgM ELISA Kit

Produktnummer ABIN930697

Human ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human .

Kurzübersicht für Anti-Cardiolipin I IgA/IgG/IgM ELISA Kit (ABIN930697)

Target: Anti-Cardiolipin I IgA/IgG/IgM

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Produktdetails

Analytische Methode: Quantitative

Produktmerkmale: ELISA kit for the detection of Cardiolipin screen IgG/IgM/IgA1 in the research laboratory
Alternative Names: Cardiolipin screen IgG/IgM/IgA1 ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end-user

Plattentyp: Pre-coated

Testdurchführung:

Highly purified cardiolipin is bound to microwells saturated with beta2glycoprotein I. Antibodies to these antigens, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG, IgM and IgA immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG, IgM resp. IgA antibodies present in the original sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Antigendetails

Target: Anti-Cardiolipin I IgA/IgG/IgM

Substanzklasse: Antibody

Hintergrund: The presence of anticardiolipin antibodies in systemic lupus erythematosus (SLE) can be related to the development of thrombosis and thrombocytopenia, in gynaecology they are supposed to cause intrauterine death or recurrent abortion. Furthermore, anticardiolipin antibodies have been found in some nonthrombotic neurological disorders like cerebrovascular insufficiency, cerebral ischemia or chorea and in myocardial infarction. Anti-cardiolipin autoantibodies are found in the immunoglobulin classes IgG, IgM and/or IgA. The determination of IgM antibodies is a valuable indicator in the diagnosis of beginning autoimmune diseases, whereas IgG antibodies will be found in progressive stages of manifested autoimmune disorders. The determination of IgA antibodies seems to have a greater importance in the African-Caribbean population.
Synonyms: Cardiolipin screen IgG/IgM/IgA1 ELISA kit.