MYH11 ELISA Kit

Produktnummer ABIN930691

Human MYH11 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human MYH11.

Kurzübersicht für MYH11 ELISA Kit (ABIN930691)

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Produktdetails MYH11 ELISA Kit

Analytische Methode: Quantitative

Produktmerkmale: ELISA kit for the detection of Sm1 in the research laboratory
Alternative Names: Smith antigen ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end-user

Plattentyp: Pre-coated

Testdurchführung:

Highly purified RNP/Sm is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu MYH11

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Andere Bezeichnung: Sm1

Hintergrund: Today the best investigated immunoreactive antigens are double stranded DNA (dsDNA), single stranded DNA, snRNP (small nuclear ribonucleoprotein particles), the complex RNP/Sm which is stabilized by ribonuleic acid as well as SSA (Ro) and SSB (La). Smith Antigen (Sm) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE).
Synonyms: Smith antigen ELISA kit.