Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

HAVCR1 ELISA Kit

Dieses Maus HAVCR1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus HAVCR1 in Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA) und Urine zu quantifizieren.
Produktnummer ABIN921114

Kurzübersicht für HAVCR1 ELISA Kit (ABIN921114)

Target

Alle HAVCR1 ELISA Kits anzeigen
HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Bindungsspezifität

AA 22-212

Reaktivität

  • 7
  • 6
  • 5
  • 2
  • 2
  • 1
Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

31.2-2000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA), Urine
  • Untere Nachweisgrenze

    31.2 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse KIM1

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: NSO
    Immunogen sequence: Y22-T212

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <2pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: Y22-T212
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Plattentyp

    Pre-coated

    Protokoll

    mouse KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for KIM1 has been precoated onto 96-well plates. Standards(NSO, Y22-T212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse KIM1 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse KIM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, tissue homogenates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse KIM1 standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 306, Standard deviation: 12.24, CV(%): 4
    • Sample 2: n=16, Mean(pg/ml): 640, Standard deviation: 35.2, CV(%): 5.5
    • Sample 3: n=16, Mean(pg/ml): 1118, Standard deviation: 69.32, CV(%): 6.2,
    • Sample 1: n=24, Mean(pg/ml): 418, Standard deviation: 24.7, CV(%): 5.9
    • Sample 2: n=24, Mean(pg/ml): 825, Standard deviation: 58.6, CV(%): 7.1
    • Sample 3: n=24, Mean(pg/ml): 1263, Standard deviation: 96, CV(%): 7.6

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle HAVCR1 ELISA Kits anzeigen

    HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

    Andere Bezeichnung

    HAVCR1

    Substanzklasse

    Virus

    Hintergrund

    Background: KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al.(1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al.(2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al.(2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.

    Synonyms: Hepatitis A virus cellular receptor 1 homolog ,Hepatitis A virus cellular receptor 1, isoform CRA_b ,Havcr1 ,mCG_117142 ,

    Full Gene Name: hepatitis A virus cellular receptor 1

    Gen-ID

    171283

    UniProt

    Q3V033
Sie sind hier:
Chat with us!