RAGE ELISA Kit

Produktnummer ABIN921104

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human RAGE. Es ist 1 Publikation verfügbar.

Kurzübersicht für RAGE ELISA Kit (ABIN921104)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Bindungsspezifität: AA 24-344

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78-5000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails RAGE ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Rage

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: Q24-A344

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q24-A344

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: human Rage ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Rage has been precoated onto 96-well plates. Standards(NSO, Q24-A344) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rage is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Rage amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Rage standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Rage standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 587, Standard deviation: 37, CV(%): 6.3
• Sample 2: n=16, Mean(pg/ml): 1624, Standard deviation: 87.7, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 3265, Standard deviation: 150.2, CV(%): 4.6,
• Sample 1: n=24, Mean(pg/ml): 592, Standard deviation: 46.77, CV(%): 7.9
• Sample 2: n=24, Mean(pg/ml): 1660, Standard deviation: 124.5, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 3347, Standard deviation: 231, CV(%): 6.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für RAGE ELISA Kit (ABIN921104)

Feng, Zhu, Zhang, Jia, Cheng, Ding, Zhu et al.: "Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via ..." in: International immunopharmacology, Vol. 15, Issue 2, pp. 206-16, (2013) (PubMed).

Details zu RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Andere Bezeichnung: AGER

Hintergrund:

Background: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as AGER. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B(NF-kappaB).

Synonyms: cDNA FLJ56412, highly similar to Advanced glycosylation end product-specific receptor ,

Full Gene Name: advanced glycosylation end product-specific receptor

Gen-ID: 177

UniProt: B4DNX3

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteine